bfish
Posts: 267
Joined: Feb. 2006
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| Quote (Lou FCD @ Nov. 10 2008,20:09) | | Room temperature, by the way, whatever that happened to be. There were certainly still a ton of maggots in the blue stuff when we removed the adults, so maybe you're right on that. We went from Monday to Monday to Monday on the lab, so it was seven days to adult removal, and another seven until we counted adults in the next generation. |
I see. Let's say room temperature is about 21 degrees C. Those flies wouldn't start emerging at all until Day 12 or 13 at that temperature, so yeah, if you were counting after 14 days, the flies were only just coming out. This can skew results for some genotypes, in that flies carrying different mutant backgrounds can tend to emerge at different rates. That's not particularly the case with white, but having a bigger n probably would have helped make your results match up a little closer to theory.
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