Joined: May 2002
Nat Struct Mol Biol. 2004 Mar 14
A novel ADP- and zinc-binding fold from function-directed in vitro evolution.
Surdo PL, Walsh MA, Sollazzo M.
|A great challenge to biologists is to create proteins with novel folds and tailored functions. As an alternative to de novo protein design, we investigated the structure of a randomly generated protein targeted to bind ATP. The crystal structure reveals a novel alpha/beta fold bound to its ligand, representing both the first protein structure derived from in vitro evolution and the first nucleotide-binding protein stabilized by a zinc ion.|