Merc
Posts: 5 Joined: June 2013
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Amazing...
John Mercer Pardon me? This was about engineering. You wrote, "Ask protein engineers how easy it is to redesign a protein to a genuinely new function." It starts with the 1999 JBC paper listed at the link Gary provided.
Biologic Institute In that paper you generate a null mutation, meaning a "loss of function" mutation. Not a new function. "As cell–cell junctions are important in morphogenesis, we generated a null mutation in the murine Af6 locus to test the hypothesis that lack of AF-6 function would cause epithelial abnormalities....
John Mercer You are mistaken. I pointed you to the 1999 **JBC** paper. You described our Current Biology paper. The JBC paper is Engineering of the myosin-I? nucleotide-binding pocket to create selective sensitivity to N 6-modified ADP analogs, JBC 274:31373.
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