Joined: May 2002
In the "just how precise would the organization of flagellum operons really have to be to get a minimal functioning flagellum?" category:
Kalir S, McClure J, Pabbaraju K, Southward C, Ronen M, Leibler S, Surette MG, Alon U.
Ordering genes in a flagella pathway by analysis of expression kinetics from living bacteria.
Science. 2001 Jun 15;292(5524):2080-3.
The recent advances in large-scale monitoring of gene expression raise the challenge of mapping systems on the basis of kinetic expression data in living cells. To address this, we measured promoter activity in the flagellar system of Escherichia coli at high accuracy and temporal resolution by means of reporter plasmids. The genes in the pathway were ordered by analysis algorithms without dependence on mutant strains. The observed temporal program of transcription was much more detailed than was previously thought and was associated with multiple steps of flagella assembly.
The precise order of transcription of the various operons is probably not essential for assembling functional flagella. This is suggested by complementation experiments in which the motility of flagella mutants was
rescued by expression of the wild-type gene from a foreign promoter (1). The detailed transcription order could, however, function to make flagella synthesis more efficient, be-cause parts are not transcribed earlier than
Edited by niiicholas on Feb. 19 2003,03:10